Rapi Stain

Rapid diff (II) stain kit is a triple stain pack for the rapid differential evaluation of blood smears, and is directly comparable to diff-quick.
1. Rapid Differential Staining of Blood Cells (Procedure time : 1 minute)
2. detecting H. pylori microorganisms
3. in cytology for interpretation of fine needle aspirate biopsies
Protocol:
1. Prepare thin blood smears on clean slide, and air dry
2. Fix by immersion in fixative solution A for 5 seconds
3. Transfer, without rinsing or drying, to red solution and stain for 5 seconds by slowly agitating the slide in the solution or immersing and withdrawing the slide several times during a 5-second period
Drain excess stain onto absorbent paper
4. Transfer slide to blue solution and repeat staining as for solution B
5. Rinse the slide briefly in buffered water (pH 6.8) or in distilled water and allow to dry
Results:
Nuclei: Varying shades of red/purple
Cytoplasm -Lymphocytes/Monocytes: Pale blue
Granules in Neutrophils: Red/Purple
Basophil granules: Blue/Black
Eosinophil granules: Bright Orange